Viable Techniques in tissue homogenization
Among the most important thing to consider when homogenizing tissues is the fibrousness and toughness of the tissue. Different homogenizers process varied tissues depending on how tight and fibrous they are. Hence, you’ll want to take on a homogenizer that is in a position to handle the sample size you intend to use. Below are some techniques used in homogenization.
Most disruption methods use some form of chemical or lysis buffer to give stability when isolating specific biomolecules. Some chemicals can be used alone to homogenize tissues effectively.
Tissue homogenizer such as surfactants and detergents aim at the biological membranes by disrupting the hydrophobic/hydrophilic interface. They are also matched with some bacteria species. Enzymes also handle the tissue walls – making it a useful first step to obtaining tissue extract. Homogenization using chemicals is preferable for small samples. The cost can be enormous for industrial-sized products.
The freeze-thawing technique is often used to disrupt mammalian tissues and bacteria. A tissue suspension is first frozen. Then, it is thawed at room temperature. The crystals formed contracts as the samples thaw. The process ruptures the cell’s membrane. While the process effectively releases recombinant cytoplasmic proteins, it requires multiple cycles. Hence, freeze-thawing is time-consuming.
Mechanical homogenization: encompasses equipment such as rotor stators and high-pressure homogenizers. Mechanical homogenization works by using pressure instead of heat. The process can easily be scaled. Hence, scientists often turn to the technique for financial and consistent results.
When it comes to ultrasonic homogenization, a combination of ultrasonic waves and cavitation is used to rupture tissues. The technique is suitable for suspended cellular structure. Ultrasonic homogenization produces a significant amount of heat. Hence, you’ll want to use the methods on tissues and molecules that will not be affected by the rising in temperature.
The use of high quality homogenizer: technique ensures you reap the benefit for both production laboratories as well as consumers alike. Currently, there are numerous companies on the market to select equipment from. However, the homogenate can be consistent and of very high quality when running through the material in excellent condition.